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KMID : 0380020050200040311
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Korean Journal of Biotechnology and Bioengineering
2005 Volume.20 No. 4 p.311 ~ p.316
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Bioinformatics based Identification and Characterization of Epoxide Hydrolase of Gordonia westfalica for the Production of Chiral Epoxides
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Lee Soo-Jung
Lee Eun-Jung
Kim Hee-Sook
Lee Eun-Yeol
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Abstract
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Epoxide hydrolases (EHs) are versatile biocatalysts for the preparation of chiral epoxides by enantioselective hydrolysis from racemic epoxides. Various microorganisms were identified to possess a EH activity by multiple sequence alignment and analysis of conserved domain sequence from genomic and megaplasmid sequence data. We successfully isolated Gordonia westfalica possessing EH activity from various microbial strains from culture type collections. G. westfalica exhibited (R)-styrene oxide preferred enantioselective hydrolysis activity. Chiral (S)-styrene oxide with high optical purity $(>\;99\%)\;ee)$ and yield of was obtained from its racemate using whole-cell of G. westfalica.
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KEYWORD
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Enantioselective hydrolysis, epoxide hydrolase, chiral epoixdes, Gordonia westfalica, multiple sequence alignment
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